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产品名称:荧光二向色镜
产品型号:

 

BrightLine荧光滤光片
  •  耀眼的荧光来自于高穿透率的滤光片 
  • 对背景光的有效阻隔带来高对比度的图像及更黑暗的背景
  • 可信赖的品质带来更长的使用寿命 – 不用担心被“烧坏”

更可提供“零像素迁移”(zero pixel shift)滤光片,用于更高要求的成像

1.多色二向色镜(BrightLine® Multi-edge Dichroic Beamsplitter)

除提供普通的二向色镜,Semrock也提供性能优良的多色二向色镜。

Filter
Nominal Edge
Wavelengths
Reflection Bands *
Transmission Bands *
Special Features
Size
440 nm
520 nm
415 - 432 nm
493 - 511 nm
449 - 483 nm
530 - 569 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
462 nm
523 nm
430 - 453 nm
508 - 512 nm
471 - 489 nm
534 - 650 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
493 nm
574 nm
456 - 480 nm
541 - 565 nm
500 - 529 nm
584 - 679 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
495 nm
605 nm
454 - 485 nm
570 - 598 nm
505 - 550 nm
620 - 675 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
497 nm
554 nm
486 - 490 nm
542 - 544 nm
420 - 471 nm
505 - 525 nm
561 - 700 nm
Designed for blocking laser wavelengths 488 nm and 543 nm
25.2 mm x 35.6 mm
x 1.1 mm
497 nm
661 nm
486 - 490 nm
646 - 648 nm
420 - 471 nm
505 - 626 nm
668 - 750 nm
Designed for blocking laser wavelengths 488 nm and 647 nm
25.2 mm x 35.6 mm
x 1.1 mm
498 nm
581 nm
486 - 490 nm
567 - 569 nm
420 - 471 nm
505 - 549 nm
587 - 700 nm
Designed for blocking laser wavelengths 488 nm and 568 nm
25.2 mm x 35.6 mm
x 1.1 mm
500 nm
646 nm
486 - 490 nm
632 - 634 nm
420 - 471 nm
505 - 613 nm
653 - 750 nm
Designed for reflecting laser wavelengths 488 nm and 633 nm
25.2 mm x 35.6 mm
x 1.1 mm
502 nm
670 nm
350 - 494 nm
641 - 660 nm
505 - 621 nm
677 - 800 nm
Designed for reflecting laser wavelengths 488 nm and 664 nm
25.2 mm x 35.6 mm
x 3.0 mm
545 nm
650 nm
532.0 nm
632.8 nm
554 - 613 nm
658 - 742 nm
Designed for dual-laser excitation (532 and 633 nm) of Cy3 and Cy5
25.2 mm x 35.6 mm
x 1.1 mm
553 nm
659 nm
542 - 544 nm
646 - 648 nm
420 - 525 nm
561 - 626 nm
668 - 750 nm
Designed for reflecting laser wavelengths 543 nm and 647 nm
25.2 mm x 35.6 mm
x 1.1 mm
555 nm
646 nm
542 - 544 nm
632 - 634 nm
420 - 525 nm
561 - 613 nm
653 - 750 nm
Designed for reflecting laser wavelengths 543 nm and 633 nm
25.2 mm x 35.6 mm
x 1.1 mm
576 nm
661 nm
567 - 569 nm
646 - 648 nm
420 - 549 nm
587 - 626 nm
668 - 750 nm
Designed for reflecting laser wavelengths 568 nm and 647 nm
25.2 mm x 35.6 mm
x 1.1 mm
579 nm
644 nm
567 - 569 nm
632 - 634 nm
420 - 549 nm
587 - 613 nm
653 - 750 nm
Designed for reflecting laser wavelengths 568 nm and 633 nm
25.2 mm x 35.6 mm
x 1.1 mm
395 nm
495 nm
610 nm
354 - 385 nm
465 - 483 nm
570 - 596 nm
403 - 446 nm
502 - 552 nm
620 - 750 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
436 nm
514 nm
604 nm
394 - 414 nm
484 - 504 nm
566 - 586 nm
446 - 468 nm
520 - 540 nm
614 - 642 nm
Designed for simultaneous excitation and detection of DAPI, FITC and Texas Red (or other blue, green and red dye combinations)
25.2 mm x 35.6 mm
x 1.1 mm
444 nm
521 nm
608 nm
420 - 430 nm
496 - 510 nm
579 - 596 nm
451 - 480 nm
530 - 561 nm
618 - 664 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
462 nm
522 nm
607 nm
403 - 453 nm
507 - 512 nm
580 - 595 nm
471 - 489 nm
532 - 560 nm
619 - 750 nm
 
25.2 x 35.6 x 3.5 mm
494 nm
540 nm
650 nm
488 nm
(s pol only)
532 nm
633 - 642 nm
500 - 519 nm
545 - 610 nm
655 - 700 nm
NBK7 substrate
25.2 x 35.6 x 3.5 mm
410 nm
504 nm
582 nm
669 nm
381 - 392 nm
475 - 495 nm
547 - 572 nm
643 - 656 nm
420 - 460 nm
510 - 531 nm
589 - 623 nm
677 - 722 nm
Designed for simultaneous excitation and detection of DAPI, FITC, TRITC or Texas Red, and Cy5 (or other blue, green, orange, and red dye combinations)
25.2 mm x 35.6 mm
x 1.1 mm

 

下列多色二向色镜可以用于透射激光,反射荧光染料发射的荧光。

Filter
Transmitted Laser Wavelengths *
Reflection Bands *
Size
³ 90% 488 nm, 532 nm, (636 - 640 nm)
³ 90% (499 - 521 nm), (543 - 623 nm), (653 - 755 nm)
25.2 mm x 35.6 mm
x 5.0 mm
³ 90% 488 nm, 568 nm, (636 - 640 nm)
³ 90% (499 - 556 nm), (580 - 622 nm), (652 - 755 nm)
25.2 mm x 35.6 mm
x 5.0 mm
³ 90% 488 nm, 532 nm, 568 nm, (636 - 640 nm)
³ 90% (499 - 521 nm), (543 - 556 nm), (580 - 622 nm), (652 - 755 nm)
25.2 mm x 35.6 mm
x 5.0 mm
³ 90% 488 nm, 532 nm, 594 nm, (636 - 640 nm)
³ 90% (499 - 521 nm), (543 - 580 nm), (608 - 623 nm), (653 - 755 nm)
25.2 mm x 35.6 mm
x 5.0 mm

2.二向色镜(BrightLine® Single-Edge Dichroic Beamsplitter)

Semrock拥有45°入射的二向色镜,该二向色镜具有极高的反射率和透过率,且对偏振特性不敏感,可以极大的提高信噪比,特别适用于荧光显微镜和荧光设备。

Filter
Nominal Edge
Wavelength
Reflection Band *
Transmission Band *
Special Features
Size
310 nm
255 - 295 nm
315 - 600 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
409 nm
344 - 404 nm
415 - 570 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
FF458-Di01-25x36
458 nm
426 - 450 nm
467 - 600 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
495 nm
442 - 488 nm
502 - 730 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
500 nm
485 - 491 nm
510 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
506 nm
446 - 500 nm
513 - 725 nm
Ideal for separating CFP and YFP emission paths in a 2-detector system
25.2 mm x 35.6 mm
x 1.1 mm
510 nm
327 - 488 nm
515 - 850 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
520 nm
488 - 512 nm
528 - 655 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
555 nm
493 - 548 nm
562 - 745 nm
Ideal for separating GFP and RFP emission paths in a 2-detector system
25.2 mm x 35.6 mm
x 1.1 mm
560 nm
485 - 545 nm
570 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
562 nm
499 - 555 nm
569 - 730 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
593 nm
530 - 585 nm
601 - 800 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
650 nm
500 - 640 nm
660 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
655 nm
470 - 645 nm
665 - 726 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
660 nm
594 - 651 nm
669 - 726 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
665 nm
350 - 650 nm
680 - 1100 nm
 
669 nm
350 - 660 nm
677 - 800 nm
 
25.2 mm x 35.6 mm
x 3.0 mm
670 nm
680 - 1080 nm
(s-polarization)
360 - 650 nm
Short-wave-pass beamsplitter
See the
Multiphoton filter page
 
685 nm
600 - 676 nm
695 - 810 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
735 nm
 350 - 720 nm
750 - 1100 nm
 
740 nm
480 - 720 nm
750 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
741nm
660 - 731.5 nm
750.5 - 810 nm
 
25.2 mm x 35.6 mm
1.1 m

3.

Semrock拥有45°入射的二向色镜,该二向色镜具有极高的反射率和透过率,且对偏振特性不敏感,可以极大的提高信噪比,特别适用于荧光显微镜和荧光设备。

Filter
Nominal Edge
Wavelength
Reflection Band *
Transmission Band *
Special Features
Size
310 nm
255 - 295 nm
315 - 600 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
409 nm
344 - 404 nm
415 - 570 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
458 nm
426 - 450 nm
467 - 600 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
495 nm
442 - 488 nm
502 - 730 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
500 nm
485 - 491 nm
510 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
506 nm
446 - 500 nm
513 - 725 nm
Ideal for separating CFP and YFP emission paths in a 2-detector system
25.2 mm x 35.6 mm
x 1.1 mm
510 nm
327 - 488 nm
515 - 850 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
520 nm
488 - 512 nm
528 - 655 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
555 nm
493 - 548 nm
562 - 745 nm
Ideal for separating GFP and RFP emission paths in a 2-detector system
25.2 mm x 35.6 mm
x 1.1 mm
560 nm
485 - 545 nm
570 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
562 nm
499 - 555 nm
569 - 730 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
593 nm
530 - 585 nm
601 - 800 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
650 nm
500 - 640 nm
660 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
655 nm
470 - 645 nm
665 - 726 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
660 nm
594 - 651 nm
669 - 726 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
665 nm
350 - 650 nm
680 - 1100 nm
 
669 nm
350 - 660 nm
677 - 800 nm
 
25.2 mm x 35.6 mm
x 3.0 mm
670 nm
680 - 1080 nm
(s-polarization)
360 - 650 nm
Short-wave-pass beamsplitter
See the
Multiphoton filter page
 
685 nm
600 - 676 nm
695 - 810 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
735 nm
 350 - 720 nm
750 - 1100 nm
 
740 nm
480 - 720 nm
750 - 825 nm
 
25.2 mm x 35.6 mm
x 1.1 mm
741nm
660 - 731.5 nm
750.5 - 810 nm
 
25.2 mm x 35.6 mm
1.1 m